The Octave^™ Chromatography System

Description

The Semba Octave™ Chromatography System is an automated liquid chromatography platform designed for preparative-scale purification of chemical and biological compounds. This bench top multicolumn system is capable of simulated moving bed chromatography (SMBC) protocols that increase productivity up to 20-fold vs. conventional single-column methods.

The Octave System carries eight column positions arranged in series and connected through a proprietary pneumatic valve array. The programmable 72-valve array contains no moving parts, occupies only 3 μl per valve, and responds within 100 ms. Fluid flow is controlled by four independent pumps. The valve and pump configuration provides ultimate flexibility in programming chromatographic protocols via the SembaPro™ Software.

The system easily accommodates continuous operation for multiple cycles of purification. All flow paths are made of metal-free biocompatible materials that are also compatible with organic solvents for chemical applications and clean-in-place solutions. With its small footprint, the Octave Chromatography System brings the high resolution and efficiency of SMBC to the bench top, packing more purification capability into this space than any other similar size chromatography instrument.

Two Octave System configurations are available that differ in their maximum pump flow rates and internal tubing systems. The Octave 10 System includes the Octave 10 Pump Module containing four precision single-piston pumps with maximum flow rates of 10 ml/min, and uses narrow 1/16” OD tubing system to minimize dead volume. The Octave 100 System is designed for larger scale applications and includes four separate dual-piston Octave 100 Pumps having maximum flow rates of 100 ml/min, and wider-bore 1/8” OD tubing system.

The Octave 100 Chromatography System: A higher-throughput system for larger scale purification of chemical and biological compounds. Flow rates up to 100 ml/min.

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The Octave 100 System includes:

• Control Module
• Four Octave 100 Pumps
• Chromatography Module 100

The Octave 100 Pump

The Octave™ 100 Pump is a dual-piston high performance pump ideal for use with the Octave 100 Chromatography System

Features

• Self-flushing PEEK™ pump heads
• Integrated prime/purge valve on each head
• LED front panel display
• Flow adjustment in 0.1 ml/min increments up to 100 ml/min
• Pressure reading and adjustable pressure limit capability
• Microprocessor advanced control
• Rapid refill mechanism to reduce pulsation
• Digital stepper motor design to prevent flow rate drift over time and temperature

The Octave™ 100 Chromatography System Components:
	Qty	Description	Catalog Number
Control Module	1	Includes electronic control interface, valve status indicator panel and all required cables	H1002K
Octave 100 Pump	4	0.1 - 100 ml/min dual-piston digital pump with Self-flush PEEK heads, biocompatible flow paths, plus RJ-11 cables and USB adapter for computer control
Chromatography Module 100	1	Includes valve block assemblies, column rack, pneumatic system, and Tubing Accessory Kit
SembaPro™ Software	1	CD or USB drive containing application software for running the system
Reservoir Tray	1	High density polyethylene tray protects top module and holds reservoir vessels

	Product Literature
	Octave System 100 Manual
	Octave 100 Pump Manual
	Octave System Brochure

Components Not Supplied

Required Components
Compressed nitrogen gas, 99.995%, dry
Gas regulator, with adjustable pressure up to 400 psi
Pneumatic hose with connector/adapter, 3/8" Male NPT at instrument
Chromatography columns (8)
Solvent reservoirs (e.g. 250 - 500 ml media bottles with GL-45 threaded tops and caps with 1/8" or 1/16" holes)

Optional Components
UV/VIS spectrometer or other detector(s) with appropriate flow cell
Fraction collector(s)

The Octave Column Stands: Versatile 8-position column holders for different sized chromatography columns.

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Rectangular Rack

Round Rack

The Octave™ Column Stand is a versatile 8-position holder for chromatography columns. The stand features a unique clamp assembly that accommodates columns up to 3.8 cm in diameter (OD). Each column is firmly held by its barrel with an integrated stainless steel spring clamp, with its outlet end supported in a slot on the lower plate. The position of the clamp assembly is infinitely adjustable along the central post by means of a locking lever that can be operated with one hand. By adjusting the height of the clamp assembly, columns up to 45 cm long can be conveniently secured.

The stand is ideal for use with Semba’s Octave Chromatography Systems, as well as for general laboratory use as a sturdy and versatile 8-position stand.

Features:

• Securely holds 8 columns up to 3.8 cm diameter x 45 cm long, minimum length 5 cm
• Spring clamps for rapid loading/unloading; no thumbscrews
• One-hand adjustable height with single locking lever
• Compact footprint; available in round or rectangular models

	Cat. No.	Product Name	Qty	Price
	H1010K	Octave™ Column Stand, Rectangular	1	$390.00
	H1011K	Octave™ Column Stand, Round	1	$390.00
	Product Literature
	Octave Column Stands Brochure

 

Accessory Products: Adapters, fittings, tubing and other tools…

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Adaptors, Fittings and Tubing
Cat. No.	Product Name	Qty	Price
A1015S	Adapter, 10-32 F to M6 F, PEEK™ (for connecting protein purification column outlets to 1/16" tubing)	Set of 8	$175.00
A1018S	Adapter, 10-32 F to M6 F, PEEK™ (for connecting protein purification column outlets to 1/16" tubing)	Set of 2	$44.00
A1040S	Adapter, ¼-28 F to M6 F, Tefzel® (for connecting protein purification column outlets to 1/8" tubing)	Set of 8	$44.00
A1035S	Adapter, ¼-28 F to 10-32 M, PEEK™ (for connecting 1/8" tubing to 1/16" column inlets and outlets)	Set of 8	$112.00
A1008S	Adapter, ShapeShifter™, ¼-28 M to 10-32 M, PEEK™ (for connecting columns directly to Octave™ valve blocks)	Set of 8	$130.00
A1001S	Fitting, One-Piece Fingertight, PEEK™, 10-32, for 1/16" OD Tubing	Set of 10	$64.00
A1002S	Nut, Super Flangeless™, PEEK™, ¼-28, for 1/16" OD Tubing	Set of 10	$32.00
A1003S	Ferrule, Super Flangeless™, PEEK™, with SST lock rings, for 1/16" OD tubing	Set of 10	$34.00
A1025S	Nut, Super Flangeless™, PEEK™, ¼-28, for 1/8" OD Tubing	Set of 10	$28.00
A1026S	Ferrule, Super Flangeless™, PEEK™, with SST lock rings, for 1/8" OD tubing	Set of 10	$29.00
A1007S	Plug, for ¼-28 flat-bottom ports, Tefzel®	Set of 10	$17.00
Back Pressure Regulators
Cat. No.	Product Name	Qty	Price
A1009K	BPR Assembly, 40 psi, with fittings for 1/16" OD tubing	ea.	$102.00
A1010K	BPR Assembly, 100 psi, with fittings for 1/16" OD tubing	ea.	$102.00
A1033K	BPR Assembly, 250 psi, with fittings for 1/16" OD tubing	ea.	$102.00
A1034K	BPR Assembly, 500 psi, with fittings for 1/16" OD tubing	ea.	$102.00
A1049K	BPR Assembly, 40 psi, with fittings for 1/8" OD tubing	ea.	$102.00
A1050K	BPR Assembly, 100 psi, with fittings for 1/8" OD tubing	ea.	$102.00
A1051K	BPR Assembly, 250 psi, with fittings for 1/8" OD tubing	ea.	$102.00
A1052K	BPR Assembly, 500 psi, with fittings for 1/8" OD tubing	ea.	$102.00
Other Tools
Cat. No.	Product Name	Qty	Price
A1047E	Tubing Cutter, Standard Polymer (for 1/16" and 1/8" OD tubing)	ea.	$23.00
A1048E	Replacement Blades (for use with A1047E)	ea.	$6.00
A1046E	Extender Tool, Aluminum (for tightening knurled fittings)	ea.	$16.00