The Octave^™ Chromatography System

Description

The Semba Octave™ Chromatography System is an automated liquid chromatography platform designed for preparative-scale purification of chemical and biological compounds. This bench top multicolumn system is capable of simulated moving bed chromatography (SMBC) protocols that increase productivity up to 20-fold vs. conventional single-column methods.

The Octave System carries eight column positions arranged in series and connected through a proprietary pneumatic valve array. The programmable 72-valve array contains no moving parts, occupies only 3 μl per valve, and responds within 100 ms. Fluid flow is controlled by four independent pumps. The valve and pump configuration provides ultimate flexibility in programming chromatographic protocols via the SembaPro™ Software.

The system easily accommodates continuous operation for multiple cycles of purification. All flow paths are made of metal-free biocompatible materials that are also compatible with organic solvents for chemical applications and clean-in-place solutions. With its small footprint, the Octave Chromatography System brings the high resolution and efficiency of SMBC to the bench top, packing more purification capability into this space than any other similar size chromatography instrument.

Two Octave System configurations are available that differ in their maximum pump flow rates and internal tubing systems. The Octave 10 System includes the Octave 10 Pump Module containing four precision single-piston pumps with maximum flow rates of 10 ml/min, and uses narrow 1/16” OD tubing system to minimize dead volume. The Octave 100 System is designed for larger scale applications and includes four separate dual-piston Octave 100 Pumps having maximum flow rates of 100 ml/min, and wider-bore 1/8” OD tubing system.

The Octave 100 Chromatography System: A higher-throughput system for larger scale purification of chemical and biological compounds. Flow rates up to 100 ml/min.

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The Octave 100 System includes:

• Control Module
• Four Octave 100 Pumps
• Chromatography Module 100

The Octave 100 Pump

The Octave™ 100 Pump is a dual-piston high performance pump ideal for use with the Octave 100 Chromatography System

Features

• Self-flushing PEEK™ pump heads
• Integrated prime/purge valve on each head
• LED front panel display
• Flow adjustment in 0.1 ml/min increments up to 100 ml/min
• Pressure reading and adjustable pressure limit capability
• Microprocessor advanced control
• Rapid refill mechanism to reduce pulsation
• Digital stepper motor design to prevent flow rate drift over time and temperature

The Octave™ 100 Chromatography System Components:
	Qty	Description	Catalog Number
Control Module	1	Includes electronic control interface, valve status indicator panel and all required cables	H1002K
Octave 100 Pump	4	0.1 - 100 ml/min dual-piston digital pump with Self-flush PEEK heads, biocompatible flow paths, plus RJ-11 cables and USB adapter for computer control
Chromatography Module 100	1	Includes valve block assemblies, column rack, pneumatic system, and Tubing Accessory Kit
SembaPro™ Software	1	CD or USB drive containing application software for running the system
Reservoir Tray	1	High density polyethylene tray protects top module and holds reservoir vessels

	Product Literature
	Octave System 100 Manual
	Octave 100 Pump Manual
	Octave System Brochure

Components Not Supplied

Required Components
Compressed nitrogen gas, 99.995%, dry
Gas regulator, with adjustable pressure up to 400 psi
Pneumatic hose with connector/adapter, 3/8" Male NPT at instrument
Chromatography columns (8)
Solvent reservoirs (e.g. 250 - 500 ml media bottles with GL-45 threaded tops and caps with 1/8" or 1/16" holes)

Optional Components
UV/VIS spectrometer or other detector(s) with appropriate flow cell
Fraction collector(s)