Chiral and Other Small Molecule Separation Columns for the Octave Chromatography System

Selecting Columns for the Small Molecule Separation
Some factors to consider when selecting columns for use with the Octave System...

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The Semba Octave™ Chromatography System can be used for SMBC purification in a variety of applications, depending on the separation medium and chemistry. The Octave System is an "open platform" permitting the use of a wide range of chromatography columns and solid phases, provided they meet some critical requirements.

An important consideration for the type of column to be used with the Semba Octave System is the expected back pressure that will be generated during operation. The total fluid back pressure limit for the instrument is 270 psi (18.6 bar, 1.86 MPa), which is due to the pressure limit of the pneumatic valve system (300 psi). Note that back pressures are additive. During typical applications no more than 3 columns are in any one zone, so in general if the back pressure generated by a single column with a given solvent and flow rate is less than 90 psi, it should be compatible with the system.

Chemical Separation

The increased productivity of SMBC allows the use of smaller columns with larger particle size and dramatically decreased plate numbers in the Octave System versus equivalent separations performed by HPLC (Perrin and Nicoud, 2001). These conditions generate lower back pressures and enable higher flow rates for preparative SMBC. In general, columns having a particle size of 10 microns or greater (ideally 15 – 30 microns) are suitable for efficient performance on the Octave System. Columns having a particle size less than 10 microns in diameter usually generate back pressures exceeding 270 psi under SMBC conditions and are not recommended for use with the Octave System.

The flow path in the Octave System is compatible with most organic solvents normally used in HPLC applications. Unsaturated halogenated hydrocarbons, e.g. methylene dichloride, trichloroethane, chloroform should be avoided.

Chiral Separation Column Sets:
See Semba's line of Astec CHIROBIOTIC™ Columns for Enantiomer Separation...

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The solid phases in CHIROBIOTIC™* columns are based on covalently bonding macrocyclic glycopeptides to a high purity 15 micron silica in such a way as to establish its stability while retaining essential components for chiral recognition.

The new Astec CHIROBIOTIC columns for SMBC feature 5 cm x 10 mm I.D column dimensions for lower back pressure and higher flow rate, making them ideal for use on the new bench-scale Semba Octave™ SMB Chromatography System. Use the corresponding 25 cm x 4.6 mm I.D. column for method development.

* Manufactured by and trademark of Supelco/Sigma-Aldrich
Features:

Rugged and reproducible for long term, reliable operation

Enantioselective for a wide range of chiral compounds

Flexible mobile phases for optimum sample solubility and operation without memory effects

CHIROBIOTIC V2 is based on bonding Vancomycin, which contains 18 chiral centers surrounding three pockets or cavities. Five aromatic ring structures bridge these strategic cavities. Hydrogen donor acceptor sites are readily available close to the ring structures.

CHIROBIOTIC T is based on bonding the amphoteric glycopeptide, Teicoplanin, which contains 23 chiral centers surrounding four pockets or cavities.

These CSPs offer mobile phase flexibility and are stable from 0-100% organic modifier.

Cat. No. Product Name Application Price

C1003S CHIROBIOTIC V2 SMB Column Set (8) Preparative $4,950

C1003E CHIROBIOTIC V2 Column
5cm x 10mm (I.D.) 15µm particles Replacement Column for SMB Set
$850

C1004E CHIROBIOTIC V2 Column
25cm x 4.6mm (I.D.) 15µm particles Method Development $850

C1005S CHIROBIOTIC T SMB Column Set (8) Preparative
$4950

C1005E CHIROBIOTIC T Column
5cm x 10mm (I.D.) 15µm particles Replacement column for SMB Set $850

C1006E CHIROBIOTIC T Column
25cm x 4.6mm (I.D.) 15µm particles Method Development $850

Ion Exchange/Ion Exclusion Column Sets:
A broad selection of media and column sizes for sugar, sugar alchohol, organic acid separations. Learn more about the full range of ion exchange columns and custom column options.

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Fructose-glucose separation using ion-exchange resins and SMBC is one of the most well-established industrial-scale SMBC applications. Relative to other separation methods, such as distillation, crystallization, and solvent extraction, ion exchange chromatography generally is faster, requires less energy, and produces less waste. The Octave™ system is the ideal tool for process research on separation of the monosaccharides of biomass, as well as for other fine chemical separations which require high-productivity and low-cost continuous ion exchange/ion exclusion chromatography processes. Semba Biosciences offers pack-to-order method development columns and SMB column sets for such applications as desalting, sugar, sugar alcohol, oligosaccharide, and organic acid separations. Please request a quote for pricing and delivery time.

10 x 100 mm Semba Columns

21.5 x 250 Columns

21 x 250 mm Semba Columns

Media	Description	Properties

Dowex™ Marathon™ MR-3 Hydrogen and Hydroxide Form	Dowex Marathon MR-3 Hydrogen and Hydroxide resin is a mixed bed ion exchange resin specially processed for condensate polishing; best for in-process and column demineralization.	Matrix: Styrene-DVB, gel Functional group: Sulfonic acid and quaternary ammonium Particle size: 550 – 660 µm Total exchange capacity, meq/ml: 1.9 H+ form, 1.0 OH- form Moisture: 54% Operating pH: 1-14 Operating Temperature: 60°C maximum

Dowex™ Monosphere™ 99Ca/320	Dowex Monosphere™ 99Ca/320 is a uniform particle size strong acid cation exchange resin for chromatographic separations of sugars and sugar alcohols. The resin is based on an 8% DVB polymer matrix with sulfonic acid functional groups, and supplied in the calcium form.	Matrix: Styrene-DVB, gel Functional group: Sulfonic acid Particle size: 320 µm Total exchange capacity, meq/ml: 1.5 (H+ form) Moisture: ~ 59% Operating pH: 1-14 Operating Temperature: 150°C maximum

Dowex™ 50WX8 Sodium Form	Dowex 50WX8 Sodium Form resin is a strongly acidic cation exchange resin for fine chemical and pharmaceutical applications. The resin is based on an 8% DVB polymer matrix with sulfonic acid functional groups, and supplied in the sodium form. This resin is ideally suited for the separation of mono-, di-, and tri-saccharides (USP L54 packing)	Matrix: Styrene-DVB, gel, 8% cross-linked Functional group: Sulfonic acid Particle size: 75 - 150µm Total exchange capacity, meq/ml: 1.7 (H+ form) Moisture: ~ 54% Operating pH: 1-14

Dowex™ 50WX8 Hydrogen Form	Dowex 50WX8 Hydrogen Form resin is a strongly acidic cation exchange resin for fine chemical and pharmaceutical applications. The resin is based on an 8% DVB polymer matrix with sulfonic acid functional groups, and supplied in the hydrogen form. This resin is used for chromatographic separations of monosaccharides, organic acids, alcohols, ketones, neutral compounds or inorganic salts (USP L17 packing).	Matrix: Styrene-DVB, gel, 8% cross-linked Functional group: Sulfonic acid Particle size: 75 - 150µm Total exchange capacity, meq/ml: 1.7 (H+ form) Moisture: ~ 54% Operating pH: 1-14

Dowex™ 50WX4 Sodium Form	Dowex 50WX4 Sodium Form resin is a strongly acidic cation exchange resin for fine chemical and pharmaceutical applications. The resin is based on an 4% DVB polymer matrix with sulfonic acid functional groups, and supplied in the sodium form. This resin can be used for chromatographic separations of oligosaccharides.	Matrix: Styrene-DVB, gel, 4% cross-linked Functional group: Sulfonic acid Particle size: 37 - 75 µm or 150 -300 µm Total exchange capacity, meq/ml: 1.1 (H+ form) Moisture: ~ 68% Operating pH: 1-14

Dowex, Marathon, and Monosphere are trademarks of The Dow Chemical Company.

Cat. No.	Product Name	Application	Price

C1003S	CHIROBIOTIC V2 SMB Column Set (8)	Preparative	$4,950
C1003E	CHIROBIOTIC V2 Column 5cm x 10mm (I.D.) 15µm particles	Replacement Column for SMB Set	$850
C1004E	CHIROBIOTIC V2 Column 25cm x 4.6mm (I.D.) 15µm particles	Method Development	$850
C1005S	CHIROBIOTIC T SMB Column Set (8)	Preparative	$4950
C1005E	CHIROBIOTIC T Column 5cm x 10mm (I.D.) 15µm particles	Replacement column for SMB Set	$850
C1006E	CHIROBIOTIC T Column 25cm x 4.6mm (I.D.) 15µm particles	Method Development	$850