Timelapse Video of Continuous Dye Separation

• SMBC Mode: 3-2-3 Isocratic
• Separation chemistry: Size exclusion
• Columns: 8 x 1 ml columns packed with Toyopearl® HW 65F, 30 - 60 micron particles
• Feed Mixture (purple color): Blue Dextran (M_r ~2 million) and cyanocobalamin (Vitamin B12; M_r 1,355)
• Extract (slow moving component) – Cyanocobalamin, red
• Raffinate (fast moving component) – Blue Dextran, blue